A Proteomic Approach for the Quantification of Posttranslational Protein Lysine Acetylation in Candida albicans

Raju Shivarathri; Manju Chauhan; Rounik Mazumdar; Phan Canh Trinh; Wolfgang Reiter; Markus Hartl; Karl Kuchler; Neeraj Chauhan

doi:10.1007/978-1-0716-2549-1_3

A Proteomic Approach for the Quantification of Posttranslational Protein Lysine Acetylation in Candida albicans

Raju Shivarathri
, Manju Chauhan
, Rounik Mazumdar
, Phan Canh Trinh
, Wolfgang Reiter
, Markus Hartl
, Karl Kuchler
, Neeraj Chauhan

Center for Discovery and Innovation

Public Health Research Institute, New York
Medical University of Vienna
University of Vienna

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Candida albicans is a normal component of the human microflora that colonizes mucosal/epithelial surfaces and the gastrointestinal tract as a commensal organism. However, in an immunocompromised host, it can cause life-threatening infections of high mortality and morbidity. Virulence as well as antifungal drug resistance of C. albicans is often regulated by posttranslational modifications (PTM) of proteins via lysine acetylation by lysine acetyltransferases. Here, we report an experimental approach using tandem mass tag (TMT) labeling for the detection and quantification of lysine acetylation of histone and nonhistone proteins in C. albicans.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	41-54
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-0716-2549-1_3
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2542
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Acetylation
Candida
Lysine acetyltransferase (KAT)
Lysine deacetylase (KDAC)
Posttranslational modification
TMT labeling

Access to Document

10.1007/978-1-0716-2549-1_3

Cite this

Shivarathri, R., Chauhan, M., Mazumdar, R., Trinh, P. C., Reiter, W., Hartl, M., Kuchler, K., & Chauhan, N. (2022). A Proteomic Approach for the Quantification of Posttranslational Protein Lysine Acetylation in Candida albicans. In Methods in Molecular Biology (pp. 41-54). (Methods in Molecular Biology; Vol. 2542). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-2549-1_3

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abstract = "Candida albicans is a normal component of the human microflora that colonizes mucosal/epithelial surfaces and the gastrointestinal tract as a commensal organism. However, in an immunocompromised host, it can cause life-threatening infections of high mortality and morbidity. Virulence as well as antifungal drug resistance of C. albicans is often regulated by posttranslational modifications (PTM) of proteins via lysine acetylation by lysine acetyltransferases. Here, we report an experimental approach using tandem mass tag (TMT) labeling for the detection and quantification of lysine acetylation of histone and nonhistone proteins in C. albicans.",

keywords = "Acetylation, Candida, Lysine acetyltransferase (KAT), Lysine deacetylase (KDAC), Posttranslational modification, TMT labeling",

author = "Raju Shivarathri and Manju Chauhan and Rounik Mazumdar and Trinh, \{Phan Canh\} and Wolfgang Reiter and Markus Hartl and Karl Kuchler and Neeraj Chauhan",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

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AU - Shivarathri, Raju

AU - Chauhan, Manju

AU - Mazumdar, Rounik

AU - Trinh, Phan Canh

AU - Reiter, Wolfgang

AU - Hartl, Markus

AU - Kuchler, Karl

AU - Chauhan, Neeraj

PY - 2022

Y1 - 2022

N2 - Candida albicans is a normal component of the human microflora that colonizes mucosal/epithelial surfaces and the gastrointestinal tract as a commensal organism. However, in an immunocompromised host, it can cause life-threatening infections of high mortality and morbidity. Virulence as well as antifungal drug resistance of C. albicans is often regulated by posttranslational modifications (PTM) of proteins via lysine acetylation by lysine acetyltransferases. Here, we report an experimental approach using tandem mass tag (TMT) labeling for the detection and quantification of lysine acetylation of histone and nonhistone proteins in C. albicans.

AB - Candida albicans is a normal component of the human microflora that colonizes mucosal/epithelial surfaces and the gastrointestinal tract as a commensal organism. However, in an immunocompromised host, it can cause life-threatening infections of high mortality and morbidity. Virulence as well as antifungal drug resistance of C. albicans is often regulated by posttranslational modifications (PTM) of proteins via lysine acetylation by lysine acetyltransferases. Here, we report an experimental approach using tandem mass tag (TMT) labeling for the detection and quantification of lysine acetylation of histone and nonhistone proteins in C. albicans.

KW - Acetylation

KW - Candida

KW - Lysine acetyltransferase (KAT)

KW - Lysine deacetylase (KDAC)

KW - Posttranslational modification

KW - TMT labeling

UR - https://www.scopus.com/pages/publications/85136584954

U2 - 10.1007/978-1-0716-2549-1_3

DO - 10.1007/978-1-0716-2549-1_3

M3 - Chapter

C2 - 36008655

AN - SCOPUS:85136584954

T3 - Methods in Molecular Biology

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EP - 54

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

A Proteomic Approach for the Quantification of Posttranslational Protein Lysine Acetylation in Candida albicans

Abstract

Publication series

UN SDGs

Keywords

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