A toxic shock syndrome toxin mutant of Staphylococcus aureus isolated by allelic replacement lacks virulence in a rabbit uterine model

Rhona Sloane; Joyce C.S. de Azavedo; John P. Arbuthnott; Patrick J. Hartigan; Barry Kreiswirth; Richard Novick; Timothy J. Foster

doi:10.1111/j.1574-6968.1991.tb04449.x

A toxic shock syndrome toxin mutant of Staphylococcus aureus isolated by allelic replacement lacks virulence in a rabbit uterine model

Rhona Sloane
, Joyce C.S. de Azavedo
, John P. Arbuthnott
, Patrick J. Hartigan
, Barry Kreiswirth
, Richard Novick
, Timothy J. Foster

Center for Discovery and Innovation

Trinity College Dublin
Public Health Research Institute, New York

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The gene coding for toxic shock syndrome toxin-1 in S. aureus was inactivated by allelic replacement in two TSS-associated strains. One mutant derived from FRI1169 (a non-enterotoxigenic strain) lacked virulence in the rabbit uterine chamber infection model. This suggests that TSST-1 is the only determinant produced by this strain that can induce the symptoms of shock in rabbits. A novel method for allelic replacement involving transduction of plasmid integrants is described.

Original language	English
Pages (from-to)	239-244
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	78
Issue number	2-3
DOIs	https://doi.org/10.1111/j.1574-6968.1991.tb04449.x
State	Published - 1 Mar 1991

Keywords

Allele replacement mutant
Animal model
Staphylococcus aureus
Toxic shock syndrome toxin

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10.1111/j.1574-6968.1991.tb04449.x

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title = "A toxic shock syndrome toxin mutant of Staphylococcus aureus isolated by allelic replacement lacks virulence in a rabbit uterine model",

abstract = "The gene coding for toxic shock syndrome toxin-1 in S. aureus was inactivated by allelic replacement in two TSS-associated strains. One mutant derived from FRI1169 (a non-enterotoxigenic strain) lacked virulence in the rabbit uterine chamber infection model. This suggests that TSST-1 is the only determinant produced by this strain that can induce the symptoms of shock in rabbits. A novel method for allelic replacement involving transduction of plasmid integrants is described.",

keywords = "Allele replacement mutant, Animal model, Staphylococcus aureus, Toxic shock syndrome toxin",

author = "Rhona Sloane and \{de Azavedo\}, \{Joyce C.S.\} and Arbuthnott, \{John P.\} and Hartigan, \{Patrick J.\} and Barry Kreiswirth and Richard Novick and Foster, \{Timothy J.\}",

year = "1991",

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doi = "10.1111/j.1574-6968.1991.tb04449.x",

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T1 - A toxic shock syndrome toxin mutant of Staphylococcus aureus isolated by allelic replacement lacks virulence in a rabbit uterine model

AU - Sloane, Rhona

AU - de Azavedo, Joyce C.S.

AU - Arbuthnott, John P.

AU - Hartigan, Patrick J.

AU - Kreiswirth, Barry

AU - Novick, Richard

AU - Foster, Timothy J.

PY - 1991/3/1

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N2 - The gene coding for toxic shock syndrome toxin-1 in S. aureus was inactivated by allelic replacement in two TSS-associated strains. One mutant derived from FRI1169 (a non-enterotoxigenic strain) lacked virulence in the rabbit uterine chamber infection model. This suggests that TSST-1 is the only determinant produced by this strain that can induce the symptoms of shock in rabbits. A novel method for allelic replacement involving transduction of plasmid integrants is described.

AB - The gene coding for toxic shock syndrome toxin-1 in S. aureus was inactivated by allelic replacement in two TSS-associated strains. One mutant derived from FRI1169 (a non-enterotoxigenic strain) lacked virulence in the rabbit uterine chamber infection model. This suggests that TSST-1 is the only determinant produced by this strain that can induce the symptoms of shock in rabbits. A novel method for allelic replacement involving transduction of plasmid integrants is described.

KW - Allele replacement mutant

KW - Animal model

KW - Staphylococcus aureus

KW - Toxic shock syndrome toxin

UR - https://www.scopus.com/pages/publications/0026090485

U2 - 10.1111/j.1574-6968.1991.tb04449.x

DO - 10.1111/j.1574-6968.1991.tb04449.x

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AN - SCOPUS:0026090485

SN - 0378-1097

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JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2-3

ER -

A toxic shock syndrome toxin mutant of Staphylococcus aureus isolated by allelic replacement lacks virulence in a rabbit uterine model

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Keywords

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