Annotation and functional clustering of circRNA expression in rhesus macaque brain during aging

Kaiyu Xu; Dong Chen; Zhengbo Wang; Jian Ma; Jian Zhou; Nanhui Chen; Longbao Lv; Yongtang Zheng; Xintian Hu; Yi Zhang; Jiali Li

doi:10.1038/s41421-018-0050-1

Annotation and functional clustering of circRNA expression in rhesus macaque brain during aging

Kaiyu Xu
, Dong Chen
, Zhengbo Wang
, Jian Ma
, Jian Zhou
, Nanhui Chen
, Longbao Lv
, Yongtang Zheng
, Xintian Hu
, Yi Zhang
, Jiali Li

School of Medicine

CAS - Kunming Institute of Zoology
University of Chinese Academy of Sciences
ABLife Inc.
Chinese Academy of Sciences

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

The abundance and function of circular RNAs (circRNAs) in mammalian brain have been reported, but their alterations in the biology of brain aging remain elusive. Here, using deep RNA profiling with linear RNA digestion by RNase R we explored a comprehensive map of changes in circRNA expression in rhesus macaque (macaca mulatta) brain in two age groups from adult (10 y) to aged (20 y) periods. Total 17,050 well expressed, stable circRNAs were identified. Cluster analysis reveals that dynamic changes in circRNA expression show the spatial-, sex- and age-biased specificities. On the basis of separate profiling of the RNAs, age-related circRNAs show differential correlation to host mRNA expression. Furthermore, two voltage-dependent L- and R-type calcium channel gene-derived circCACNA2D1 and circCACNA1E negatively regulate their host mRNA expression. Our results demonstrate the power of changes in circRNA expression to reveal insights into a potentially circRNA-mediated regulatory mechanism underlying the biology of brain aging.

Original language	English
Article number	48
Journal	Cell Discovery
Volume	4
Issue number	1
DOIs	https://doi.org/10.1038/s41421-018-0050-1
State	Published - 1 Dec 2018

Access to Document

10.1038/s41421-018-0050-1

Cite this

@article{43e1c8895b7d4077b8421777a3cbfa83,

title = "Annotation and functional clustering of circRNA expression in rhesus macaque brain during aging",

abstract = "The abundance and function of circular RNAs (circRNAs) in mammalian brain have been reported, but their alterations in the biology of brain aging remain elusive. Here, using deep RNA profiling with linear RNA digestion by RNase R we explored a comprehensive map of changes in circRNA expression in rhesus macaque (macaca mulatta) brain in two age groups from adult (10 y) to aged (20 y) periods. Total 17,050 well expressed, stable circRNAs were identified. Cluster analysis reveals that dynamic changes in circRNA expression show the spatial-, sex- and age-biased specificities. On the basis of separate profiling of the RNAs, age-related circRNAs show differential correlation to host mRNA expression. Furthermore, two voltage-dependent L- and R-type calcium channel gene-derived circCACNA2D1 and circCACNA1E negatively regulate their host mRNA expression. Our results demonstrate the power of changes in circRNA expression to reveal insights into a potentially circRNA-mediated regulatory mechanism underlying the biology of brain aging.",

author = "Kaiyu Xu and Dong Chen and Zhengbo Wang and Jian Ma and Jian Zhou and Nanhui Chen and Longbao Lv and Yongtang Zheng and Xintian Hu and Yi Zhang and Jiali Li",

note = "Publisher Copyright: {\textcopyright} 2018, The Author(s).",

year = "2018",

month = dec,

day = "1",

doi = "10.1038/s41421-018-0050-1",

language = "English",

volume = "4",

journal = "Cell Discovery",

issn = "2056-5968",

publisher = "Springer Nature",

number = "1",

}

TY - JOUR

T1 - Annotation and functional clustering of circRNA expression in rhesus macaque brain during aging

AU - Xu, Kaiyu

AU - Chen, Dong

AU - Wang, Zhengbo

AU - Ma, Jian

AU - Zhou, Jian

AU - Chen, Nanhui

AU - Lv, Longbao

AU - Zheng, Yongtang

AU - Hu, Xintian

AU - Zhang, Yi

AU - Li, Jiali

PY - 2018/12/1

Y1 - 2018/12/1

N2 - The abundance and function of circular RNAs (circRNAs) in mammalian brain have been reported, but their alterations in the biology of brain aging remain elusive. Here, using deep RNA profiling with linear RNA digestion by RNase R we explored a comprehensive map of changes in circRNA expression in rhesus macaque (macaca mulatta) brain in two age groups from adult (10 y) to aged (20 y) periods. Total 17,050 well expressed, stable circRNAs were identified. Cluster analysis reveals that dynamic changes in circRNA expression show the spatial-, sex- and age-biased specificities. On the basis of separate profiling of the RNAs, age-related circRNAs show differential correlation to host mRNA expression. Furthermore, two voltage-dependent L- and R-type calcium channel gene-derived circCACNA2D1 and circCACNA1E negatively regulate their host mRNA expression. Our results demonstrate the power of changes in circRNA expression to reveal insights into a potentially circRNA-mediated regulatory mechanism underlying the biology of brain aging.

AB - The abundance and function of circular RNAs (circRNAs) in mammalian brain have been reported, but their alterations in the biology of brain aging remain elusive. Here, using deep RNA profiling with linear RNA digestion by RNase R we explored a comprehensive map of changes in circRNA expression in rhesus macaque (macaca mulatta) brain in two age groups from adult (10 y) to aged (20 y) periods. Total 17,050 well expressed, stable circRNAs were identified. Cluster analysis reveals that dynamic changes in circRNA expression show the spatial-, sex- and age-biased specificities. On the basis of separate profiling of the RNAs, age-related circRNAs show differential correlation to host mRNA expression. Furthermore, two voltage-dependent L- and R-type calcium channel gene-derived circCACNA2D1 and circCACNA1E negatively regulate their host mRNA expression. Our results demonstrate the power of changes in circRNA expression to reveal insights into a potentially circRNA-mediated regulatory mechanism underlying the biology of brain aging.

UR - https://www.scopus.com/pages/publications/85053427341

U2 - 10.1038/s41421-018-0050-1

DO - 10.1038/s41421-018-0050-1

M3 - Article

AN - SCOPUS:85053427341

SN - 2056-5968

VL - 4

JO - Cell Discovery

JF - Cell Discovery

IS - 1

M1 - 48

ER -

Annotation and functional clustering of circRNA expression in rhesus macaque brain during aging

Abstract

Access to Document

Other files and links

Fingerprint

Cite this