Characterization of angiotensin-(1-7) receptor subtype in mesenteric arteries

Liomar A.A. Neves; David B. Averill; Carlos M. Ferrario; Mark C. Chappell; Judy L. Aschner; Michael P. Walkup; K. Bridget Brosnihan

doi:10.1016/S0196-9781(03)00062-7

Characterization of angiotensin-(1-7) receptor subtype in mesenteric arteries

Liomar A.A. Neves
, David B. Averill
, Carlos M. Ferrario
, Mark C. Chappell
, Judy L. Aschner
, Michael P. Walkup
, K. Bridget Brosnihan

School of Medicine

Wake Forest University

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Mesenteric arteries from male Sprague-Dawley rats were mounted in a pressurized myograph system. Ang-(1-7) concentration-dependent responses were determined in arteries preconstricted with endothelin-1 (10^-7M). The receptor(s) mediating the Ang-(1-7) evoked dilation were investigated by pretreating the mesenteric arteries with specific antagonists of Ang-(1-7), AT₁ or AT₂ receptors. The effects of Ang-(3-8) and Ang-(3-7) were also determined. Ang-(1-7) caused a concentration-dependent dilation (EC₅₀: 0.95nM) that was blocked by the selective Ang-(1-7) receptor antagonist D-[Ala⁷]-Ang-(1-7). Administration of a specific antagonist to the AT₂ receptor (PD123319) had no effect. On the other hand, losartan and CV-11974 attenuated the Ang-(1-7) effect. These results demonstrate that Ang-(1-7) elicits potent dilation of mesenteric resistance vessels mediated by a D-[Ala⁷]-Ang-(1-7) sensitive site that is also sensitive to losartan and CV-11974.

Original language	English
Pages (from-to)	455-462
Number of pages	8
Journal	Peptides
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/S0196-9781(03)00062-7
State	Published - 1 Mar 2003

Keywords

Angiotensin receptors
Hypertension
Renin-angiotensin system
Vasodilation

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title = "Characterization of angiotensin-(1-7) receptor subtype in mesenteric arteries",

abstract = "Mesenteric arteries from male Sprague-Dawley rats were mounted in a pressurized myograph system. Ang-(1-7) concentration-dependent responses were determined in arteries preconstricted with endothelin-1 (10-7M). The receptor(s) mediating the Ang-(1-7) evoked dilation were investigated by pretreating the mesenteric arteries with specific antagonists of Ang-(1-7), AT1 or AT2 receptors. The effects of Ang-(3-8) and Ang-(3-7) were also determined. Ang-(1-7) caused a concentration-dependent dilation (EC50: 0.95nM) that was blocked by the selective Ang-(1-7) receptor antagonist D-[Ala7]-Ang-(1-7). Administration of a specific antagonist to the AT2 receptor (PD123319) had no effect. On the other hand, losartan and CV-11974 attenuated the Ang-(1-7) effect. These results demonstrate that Ang-(1-7) elicits potent dilation of mesenteric resistance vessels mediated by a D-[Ala7]-Ang-(1-7) sensitive site that is also sensitive to losartan and CV-11974.",

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author = "Neves, \{Liomar A.A.\} and Averill, \{David B.\} and Ferrario, \{Carlos M.\} and Chappell, \{Mark C.\} and Aschner, \{Judy L.\} and Walkup, \{Michael P.\} and Brosnihan, \{K. Bridget\}",

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AU - Neves, Liomar A.A.

AU - Averill, David B.

AU - Ferrario, Carlos M.

AU - Chappell, Mark C.

AU - Aschner, Judy L.

AU - Walkup, Michael P.

AU - Brosnihan, K. Bridget

PY - 2003/3/1

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N2 - Mesenteric arteries from male Sprague-Dawley rats were mounted in a pressurized myograph system. Ang-(1-7) concentration-dependent responses were determined in arteries preconstricted with endothelin-1 (10-7M). The receptor(s) mediating the Ang-(1-7) evoked dilation were investigated by pretreating the mesenteric arteries with specific antagonists of Ang-(1-7), AT1 or AT2 receptors. The effects of Ang-(3-8) and Ang-(3-7) were also determined. Ang-(1-7) caused a concentration-dependent dilation (EC50: 0.95nM) that was blocked by the selective Ang-(1-7) receptor antagonist D-[Ala7]-Ang-(1-7). Administration of a specific antagonist to the AT2 receptor (PD123319) had no effect. On the other hand, losartan and CV-11974 attenuated the Ang-(1-7) effect. These results demonstrate that Ang-(1-7) elicits potent dilation of mesenteric resistance vessels mediated by a D-[Ala7]-Ang-(1-7) sensitive site that is also sensitive to losartan and CV-11974.

AB - Mesenteric arteries from male Sprague-Dawley rats were mounted in a pressurized myograph system. Ang-(1-7) concentration-dependent responses were determined in arteries preconstricted with endothelin-1 (10-7M). The receptor(s) mediating the Ang-(1-7) evoked dilation were investigated by pretreating the mesenteric arteries with specific antagonists of Ang-(1-7), AT1 or AT2 receptors. The effects of Ang-(3-8) and Ang-(3-7) were also determined. Ang-(1-7) caused a concentration-dependent dilation (EC50: 0.95nM) that was blocked by the selective Ang-(1-7) receptor antagonist D-[Ala7]-Ang-(1-7). Administration of a specific antagonist to the AT2 receptor (PD123319) had no effect. On the other hand, losartan and CV-11974 attenuated the Ang-(1-7) effect. These results demonstrate that Ang-(1-7) elicits potent dilation of mesenteric resistance vessels mediated by a D-[Ala7]-Ang-(1-7) sensitive site that is also sensitive to losartan and CV-11974.

KW - Angiotensin receptors

KW - Hypertension

KW - Renin-angiotensin system

KW - Vasodilation

UR - https://www.scopus.com/pages/publications/0038066401

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VL - 24

SP - 455

EP - 462

JO - Peptides

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IS - 3

ER -

Characterization of angiotensin-(1-7) receptor subtype in mesenteric arteries

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