Coidentification of mcr-4.3 and blaNDM-1 in a clinical enterobacter cloacae isolate from China

Bhakti Chavda; Jingnan Lv; Mengyun Hou; Kalyan D. Chavda; Barry N. Kreiswirth; Youjun Feng; Liang Chen; Fangyou Yu

doi:10.1128/AAC.00649-18

Coidentification of mcr-4.3 and blaNDM-1 in a clinical enterobacter cloacae isolate from China

Bhakti Chavda
, Jingnan Lv
, Mengyun Hou
, Kalyan D. Chavda
, Barry N. Kreiswirth
, Youjun Feng
, Liang Chen
, Fangyou Yu

Center for Discovery and Innovation

Rutgers - The State University of New Jersey, Newark
Wenzhou Medical University
Zhejiang University
Tongji University

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

We describe the first report of a clinical colistin-resistant ST84 Enterobacter cloacae isolate coharboring mcr-4.3 (previously named mcr-4.2) and blaNDM-1 from a patient in China. The blaNDM-1-harboring IncX3 plasmid and the novel mcr-4.3-harboring ColE plasmid were completely sequenced. Although this isolate showed a high level of resistance to colistin, mcr-4.3 plasmid transformation, gene subcloning, susceptibility testing, and lipid A matrix-assisted laser desorption ionization mass spectrometry analysis indicated that mcr-4.3 itself does not confer resistance to colistin.

Original language	English
Article number	e00649
Journal	Antimicrobial Agents and Chemotherapy
Volume	62
Issue number	10
DOIs	https://doi.org/10.1128/AAC.00649-18
State	Published - Oct 2018

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Carbapenem resistant
Colistin
Mcr-4
NDM-1
Plasmid

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10.1128/AAC.00649-18

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title = "Coidentification of mcr-4.3 and blaNDM-1 in a clinical enterobacter cloacae isolate from China",

abstract = "We describe the first report of a clinical colistin-resistant ST84 Enterobacter cloacae isolate coharboring mcr-4.3 (previously named mcr-4.2) and blaNDM-1 from a patient in China. The blaNDM-1-harboring IncX3 plasmid and the novel mcr-4.3-harboring ColE plasmid were completely sequenced. Although this isolate showed a high level of resistance to colistin, mcr-4.3 plasmid transformation, gene subcloning, susceptibility testing, and lipid A matrix-assisted laser desorption ionization mass spectrometry analysis indicated that mcr-4.3 itself does not confer resistance to colistin.",

keywords = "Carbapenem resistant, Colistin, Mcr-4, NDM-1, Plasmid",

author = "Bhakti Chavda and Jingnan Lv and Mengyun Hou and Chavda, \{Kalyan D.\} and Kreiswirth, \{Barry N.\} and Youjun Feng and Liang Chen and Fangyou Yu",

note = "Publisher Copyright: {\textcopyright} 2018 American Society for Microbiology.",

year = "2018",

month = oct,

doi = "10.1128/AAC.00649-18",

language = "English",

volume = "62",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

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T1 - Coidentification of mcr-4.3 and blaNDM-1 in a clinical enterobacter cloacae isolate from China

AU - Chavda, Bhakti

AU - Lv, Jingnan

AU - Hou, Mengyun

AU - Chavda, Kalyan D.

AU - Kreiswirth, Barry N.

AU - Feng, Youjun

AU - Chen, Liang

AU - Yu, Fangyou

PY - 2018/10

Y1 - 2018/10

N2 - We describe the first report of a clinical colistin-resistant ST84 Enterobacter cloacae isolate coharboring mcr-4.3 (previously named mcr-4.2) and blaNDM-1 from a patient in China. The blaNDM-1-harboring IncX3 plasmid and the novel mcr-4.3-harboring ColE plasmid were completely sequenced. Although this isolate showed a high level of resistance to colistin, mcr-4.3 plasmid transformation, gene subcloning, susceptibility testing, and lipid A matrix-assisted laser desorption ionization mass spectrometry analysis indicated that mcr-4.3 itself does not confer resistance to colistin.

AB - We describe the first report of a clinical colistin-resistant ST84 Enterobacter cloacae isolate coharboring mcr-4.3 (previously named mcr-4.2) and blaNDM-1 from a patient in China. The blaNDM-1-harboring IncX3 plasmid and the novel mcr-4.3-harboring ColE plasmid were completely sequenced. Although this isolate showed a high level of resistance to colistin, mcr-4.3 plasmid transformation, gene subcloning, susceptibility testing, and lipid A matrix-assisted laser desorption ionization mass spectrometry analysis indicated that mcr-4.3 itself does not confer resistance to colistin.

KW - Carbapenem resistant

KW - Colistin

KW - Mcr-4

KW - NDM-1

KW - Plasmid

UR - https://www.scopus.com/pages/publications/85053940009

U2 - 10.1128/AAC.00649-18

DO - 10.1128/AAC.00649-18

M3 - Article

C2 - 30038043

AN - SCOPUS:85053940009

SN - 0066-4804

VL - 62

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 10

M1 - e00649

ER -

Coidentification of mcr-4.3 and blaNDM-1 in a clinical enterobacter cloacae isolate from China

Abstract

UN SDGs

Keywords

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