Colocation of Lipids, Drugs, and Metal Biomarkers Using Spatially Resolved Lipidomics with Elemental Mapping

Holly May Lewis; Catia Costa; Véronique Dartois; Firat Kaya; Mark Chambers; Janella De Jesus; Vladimir Palitsin; Roger Webb; Melanie J. Bailey

doi:10.1021/acs.analchem.2c01940

Colocation of Lipids, Drugs, and Metal Biomarkers Using Spatially Resolved Lipidomics with Elemental Mapping

Holly May Lewis
, Catia Costa
, Véronique Dartois
, Firat Kaya
, Mark Chambers
, Janella De Jesus
, Vladimir Palitsin
, Roger Webb
, Melanie J. Bailey

Center for Discovery and Innovation

University of Surrey
Hackensack Meridian School of Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Elemental imaging is widely used for imaging cells and tissues but rarely in combination with organic mass spectrometry, which can be used to profile lipids and measure drug concentrations. Here, we demonstrate how elemental imaging and a new method for spatially resolved lipidomics (DAPNe-LC-MS, based on capillary microsampling and liquid chromatography mass spectrometry) can be used in combination to probe the relationship between metals, drugs, and lipids in discrete areas of tissues. This new method for spatial lipidomics, reported here for the first time, has been applied to rabbit lung tissues containing a lesion (caseous granuloma) caused by tuberculosis infection. We demonstrate how elemental imaging with spatially resolved lipidomics can be used to probe the association between ion accumulation and lipid profiles and verify local drug distribution.

Original language	English
Pages (from-to)	11798-11806
Number of pages	9
Journal	Analytical Chemistry
Volume	94
Issue number	34
DOIs	https://doi.org/10.1021/acs.analchem.2c01940
State	Published - 30 Aug 2022

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10.1021/acs.analchem.2c01940

Cite this

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title = "Colocation of Lipids, Drugs, and Metal Biomarkers Using Spatially Resolved Lipidomics with Elemental Mapping",

abstract = "Elemental imaging is widely used for imaging cells and tissues but rarely in combination with organic mass spectrometry, which can be used to profile lipids and measure drug concentrations. Here, we demonstrate how elemental imaging and a new method for spatially resolved lipidomics (DAPNe-LC-MS, based on capillary microsampling and liquid chromatography mass spectrometry) can be used in combination to probe the relationship between metals, drugs, and lipids in discrete areas of tissues. This new method for spatial lipidomics, reported here for the first time, has been applied to rabbit lung tissues containing a lesion (caseous granuloma) caused by tuberculosis infection. We demonstrate how elemental imaging with spatially resolved lipidomics can be used to probe the association between ion accumulation and lipid profiles and verify local drug distribution.",

author = "Lewis, \{Holly May\} and Catia Costa and V{\'e}ronique Dartois and Firat Kaya and Mark Chambers and \{De Jesus\}, Janella and Vladimir Palitsin and Roger Webb and Bailey, \{Melanie J.\}",

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doi = "10.1021/acs.analchem.2c01940",

language = "English",

volume = "94",

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T1 - Colocation of Lipids, Drugs, and Metal Biomarkers Using Spatially Resolved Lipidomics with Elemental Mapping

AU - Lewis, Holly May

AU - Costa, Catia

AU - Dartois, Véronique

AU - Kaya, Firat

AU - Chambers, Mark

AU - De Jesus, Janella

AU - Palitsin, Vladimir

AU - Webb, Roger

AU - Bailey, Melanie J.

PY - 2022/8/30

Y1 - 2022/8/30

N2 - Elemental imaging is widely used for imaging cells and tissues but rarely in combination with organic mass spectrometry, which can be used to profile lipids and measure drug concentrations. Here, we demonstrate how elemental imaging and a new method for spatially resolved lipidomics (DAPNe-LC-MS, based on capillary microsampling and liquid chromatography mass spectrometry) can be used in combination to probe the relationship between metals, drugs, and lipids in discrete areas of tissues. This new method for spatial lipidomics, reported here for the first time, has been applied to rabbit lung tissues containing a lesion (caseous granuloma) caused by tuberculosis infection. We demonstrate how elemental imaging with spatially resolved lipidomics can be used to probe the association between ion accumulation and lipid profiles and verify local drug distribution.

AB - Elemental imaging is widely used for imaging cells and tissues but rarely in combination with organic mass spectrometry, which can be used to profile lipids and measure drug concentrations. Here, we demonstrate how elemental imaging and a new method for spatially resolved lipidomics (DAPNe-LC-MS, based on capillary microsampling and liquid chromatography mass spectrometry) can be used in combination to probe the relationship between metals, drugs, and lipids in discrete areas of tissues. This new method for spatial lipidomics, reported here for the first time, has been applied to rabbit lung tissues containing a lesion (caseous granuloma) caused by tuberculosis infection. We demonstrate how elemental imaging with spatially resolved lipidomics can be used to probe the association between ion accumulation and lipid profiles and verify local drug distribution.

UR - https://www.scopus.com/pages/publications/85136521687

U2 - 10.1021/acs.analchem.2c01940

DO - 10.1021/acs.analchem.2c01940

M3 - Article

AN - SCOPUS:85136521687

SN - 0003-2700

VL - 94

SP - 11798

EP - 11806

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 34

ER -

Colocation of Lipids, Drugs, and Metal Biomarkers Using Spatially Resolved Lipidomics with Elemental Mapping

Abstract

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