Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization

N. Dickgiesser; B. N. Kreiswirth

doi:10.1128/AAC.29.5.930

Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization

N. Dickgiesser
, B. N. Kreiswirth

Heidelberg University

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A method is described for identification of the genes conferring aminoglycoside resistance in Staphylococcus aureus by dot-blot and Southern blot techniques. As radioactive probes, fragments of plasmids pAT48, pUBH₂, and pH13, carrying the genes for an aminocyclitol-3'-phosphotransferase, an aminocyclitol-4'-adenylyltransferase, and an aminocyclitol-2''-phosphotransferase-aminocyclitol-6'-acetyltransferase, respectively, were used.

Original language	English
Pages (from-to)	930-932
Number of pages	3
Journal	Antimicrobial Agents and Chemotherapy
Volume	29
Issue number	5
DOIs	https://doi.org/10.1128/AAC.29.5.930
State	Published - 1986

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10.1128/AAC.29.5.930

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title = "Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization",

abstract = "A method is described for identification of the genes conferring aminoglycoside resistance in Staphylococcus aureus by dot-blot and Southern blot techniques. As radioactive probes, fragments of plasmids pAT48, pUBH2, and pH13, carrying the genes for an aminocyclitol-3'-phosphotransferase, an aminocyclitol-4'-adenylyltransferase, and an aminocyclitol-2''-phosphotransferase-aminocyclitol-6'-acetyltransferase, respectively, were used.",

author = "N. Dickgiesser and Kreiswirth, \{B. N.\}",

year = "1986",

doi = "10.1128/AAC.29.5.930",

language = "English",

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journal = "Antimicrobial Agents and Chemotherapy",

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publisher = "American Society for Microbiology",

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T1 - Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization

AU - Dickgiesser, N.

AU - Kreiswirth, B. N.

PY - 1986

Y1 - 1986

N2 - A method is described for identification of the genes conferring aminoglycoside resistance in Staphylococcus aureus by dot-blot and Southern blot techniques. As radioactive probes, fragments of plasmids pAT48, pUBH2, and pH13, carrying the genes for an aminocyclitol-3'-phosphotransferase, an aminocyclitol-4'-adenylyltransferase, and an aminocyclitol-2''-phosphotransferase-aminocyclitol-6'-acetyltransferase, respectively, were used.

AB - A method is described for identification of the genes conferring aminoglycoside resistance in Staphylococcus aureus by dot-blot and Southern blot techniques. As radioactive probes, fragments of plasmids pAT48, pUBH2, and pH13, carrying the genes for an aminocyclitol-3'-phosphotransferase, an aminocyclitol-4'-adenylyltransferase, and an aminocyclitol-2''-phosphotransferase-aminocyclitol-6'-acetyltransferase, respectively, were used.

UR - https://www.scopus.com/pages/publications/0022625043

U2 - 10.1128/AAC.29.5.930

DO - 10.1128/AAC.29.5.930

M3 - Article

C2 - 3729351

AN - SCOPUS:0022625043

SN - 0066-4804

VL - 29

SP - 930

EP - 932

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 5

ER -

Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization

Abstract

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