Distribution of CD103                         +                          CD8                         +                          T cell infiltration in colorectal cancer tissues and its clinical significance

Huimin Lu; Yan Wang; Lujun Chen; Jingting Jiang; Binfeng Lu

doi:10.3872/j.issn.1007-385X.2019.01.009

Distribution of CD103 ⁺ CD8 ⁺ T cell infiltration in colorectal cancer tissues and its clinical significance

Huimin Lu
, Yan Wang
, Lujun Chen
, Jingting Jiang
, Binfeng Lu

Center for Discovery and Innovation

Soochow University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Objective: To investigate the degree and distribution of tissue-resident CD8 ⁺ T cell (CD103 ⁺ CD8 ⁺ T cells) infiltration in colorectal cancer (CRC) tissues, and to analyze its relationship to patients’clinicopathological features and prognosis. Methods: Tissue chips of 88 cases of colon cancer tissues (No.HColA180Su14) and 77 cases of rectal cancer tissues (No. HRec-Ade180Sur-03) were obtained from Shanghai Outdo Biotech Co., Ltd. Immunofluorescence staining was performed to examine the infiltration pattern and degree of CD103 ⁺ CD8 ⁺ T cells in the collected CRC tissues and their para-cancerous tissues. Wilcoxon rank test was used to compare CD103 ⁺ CD8 ⁺ T cell infiltration degree in CRC tissues and the para-cancerous tissues. Chi-square test was used to analyze the relationship between CD103 ⁺ CD8 ⁺ T cell infiltration in CRC and patients’clinicopathological features. Kaplan-Meier survival analysis was conducted to explore the correlation between CD103 ⁺ CD8 ⁺ T cell infiltration and patients’prognosis. Cox model was applied to analyze the correlation between different clinical parameters and patients’prognosis. Results: CD103 ⁺ CD8 ⁺ T cell infiltration presented no significant differences between CRC and para-cancer tissues (P>0.05). Patients with distant metastasis had significantly lower CD103 ⁺ CD8 ⁺ T cell infiltration rate than patients without distant metastasis (P<0.01). There was no significant correlation between the infiltration of CD103 ⁺ CD8 ⁺ T cells and other clinicopathological features (P>0.05). Kaplan-Meier survival analysis showed that the overall survival (OS) of patients with high CD103 ⁺ CD8 ⁺ T cell infiltration was significantly longer than that of the patients with low infiltration (54.42% vs 25.00%, P<0.05). Multivariate Cox model analysis indicated that pathological grade (P<0.01) and high CD103 ⁺ CD8 ⁺ T cell infiltration (P<0.05) were independent prognostic factors for CRC. Conclusion：CD103+CD8+T cell infiltration in CRC is associated with patients’prognosis, suggesting that CD103 ⁺ CD8 ⁺ T cell plays an important role in the initiation and development of CRC.

Original language	English
Article number	1007-385X(2019)01-0050-08
Pages (from-to)	50-57
Number of pages	8
Journal	Chinese Journal of Cancer Biotherapy
Volume	26
Issue number	1
DOIs	https://doi.org/10.3872/j.issn.1007-385X.2019.01.009
State	Published - Jan 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

CD103 CD8 T cell
Colorectal cancer
Prognosis
Tissue microarray

Access to Document

10.3872/j.issn.1007-385X.2019.01.009

Cite this

Lu, H., Wang, Y., Chen, L., Jiang, J., & Lu, B. (2019). Distribution of CD103 ⁺ CD8 ⁺ T cell infiltration in colorectal cancer tissues and its clinical significance Chinese Journal of Cancer Biotherapy, 26(1), 50-57. Article 1007-385X(2019)01-0050-08. https://doi.org/10.3872/j.issn.1007-385X.2019.01.009

Lu, Huimin ; Wang, Yan ; Chen, Lujun et al. / Distribution of CD103 ⁺ CD8 ⁺ T cell infiltration in colorectal cancer tissues and its clinical significance In: Chinese Journal of Cancer Biotherapy. 2019 ; Vol. 26, No. 1. pp. 50-57.

@article{64166cf33e3c444d968f67487754c93b,

title = " Distribution of CD103 + CD8 + T cell infiltration in colorectal cancer tissues and its clinical significance ",

abstract = " Objective: To investigate the degree and distribution of tissue-resident CD8 + T cell (CD103 + CD8 + T cells) infiltration in colorectal cancer (CRC) tissues, and to analyze its relationship to patients{\textquoteright}clinicopathological features and prognosis. Methods: Tissue chips of 88 cases of colon cancer tissues (No.HColA180Su14) and 77 cases of rectal cancer tissues (No. HRec-Ade180Sur-03) were obtained from Shanghai Outdo Biotech Co., Ltd. Immunofluorescence staining was performed to examine the infiltration pattern and degree of CD103 + CD8 + T cells in the collected CRC tissues and their para-cancerous tissues. Wilcoxon rank test was used to compare CD103 + CD8 + T cell infiltration degree in CRC tissues and the para-cancerous tissues. Chi-square test was used to analyze the relationship between CD103 + CD8 + T cell infiltration in CRC and patients{\textquoteright}clinicopathological features. Kaplan-Meier survival analysis was conducted to explore the correlation between CD103 + CD8 + T cell infiltration and patients{\textquoteright}prognosis. Cox model was applied to analyze the correlation between different clinical parameters and patients{\textquoteright}prognosis. Results: CD103 + CD8 + T cell infiltration presented no significant differences between CRC and para-cancer tissues (P>0.05). Patients with distant metastasis had significantly lower CD103 + CD8 + T cell infiltration rate than patients without distant metastasis (P<0.01). There was no significant correlation between the infiltration of CD103 + CD8 + T cells and other clinicopathological features (P>0.05). Kaplan-Meier survival analysis showed that the overall survival (OS) of patients with high CD103 + CD8 + T cell infiltration was significantly longer than that of the patients with low infiltration (54.42\% vs 25.00\%, P<0.05). Multivariate Cox model analysis indicated that pathological grade (P<0.01) and high CD103 + CD8 + T cell infiltration (P<0.05) were independent prognostic factors for CRC. Conclusion：CD103+CD8+T cell infiltration in CRC is associated with patients{\textquoteright}prognosis, suggesting that CD103 + CD8 + T cell plays an important role in the initiation and development of CRC.",

keywords = "CD103 CD8 T cell, Colorectal cancer, Prognosis, Tissue microarray",

author = "Huimin Lu and Yan Wang and Lujun Chen and Jingting Jiang and Binfeng Lu",

year = "2019",

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doi = "10.3872/j.issn.1007-385X.2019.01.009",

language = "English",

volume = "26",

pages = "50--57",

journal = "Chinese Journal of Cancer Biotherapy",

issn = "1007-385X",

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Lu, H, Wang, Y, Chen, L, Jiang, J & Lu, B 2019, ' Distribution of CD103 ⁺ CD8 ⁺ T cell infiltration in colorectal cancer tissues and its clinical significance ', Chinese Journal of Cancer Biotherapy, vol. 26, no. 1, 1007-385X(2019)01-0050-08, pp. 50-57. https://doi.org/10.3872/j.issn.1007-385X.2019.01.009

Distribution of CD103 ⁺ CD8 ⁺ T cell infiltration in colorectal cancer tissues and its clinical significance . / Lu, Huimin; Wang, Yan; Chen, Lujun et al.
In: Chinese Journal of Cancer Biotherapy, Vol. 26, No. 1, 1007-385X(2019)01-0050-08, 01.2019, p. 50-57.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Distribution of CD103 + CD8 + T cell infiltration in colorectal cancer tissues and its clinical significance

AU - Lu, Huimin

AU - Wang, Yan

AU - Chen, Lujun

AU - Jiang, Jingting

AU - Lu, Binfeng

PY - 2019/1

Y1 - 2019/1

N2 - Objective: To investigate the degree and distribution of tissue-resident CD8 + T cell (CD103 + CD8 + T cells) infiltration in colorectal cancer (CRC) tissues, and to analyze its relationship to patients’clinicopathological features and prognosis. Methods: Tissue chips of 88 cases of colon cancer tissues (No.HColA180Su14) and 77 cases of rectal cancer tissues (No. HRec-Ade180Sur-03) were obtained from Shanghai Outdo Biotech Co., Ltd. Immunofluorescence staining was performed to examine the infiltration pattern and degree of CD103 + CD8 + T cells in the collected CRC tissues and their para-cancerous tissues. Wilcoxon rank test was used to compare CD103 + CD8 + T cell infiltration degree in CRC tissues and the para-cancerous tissues. Chi-square test was used to analyze the relationship between CD103 + CD8 + T cell infiltration in CRC and patients’clinicopathological features. Kaplan-Meier survival analysis was conducted to explore the correlation between CD103 + CD8 + T cell infiltration and patients’prognosis. Cox model was applied to analyze the correlation between different clinical parameters and patients’prognosis. Results: CD103 + CD8 + T cell infiltration presented no significant differences between CRC and para-cancer tissues (P>0.05). Patients with distant metastasis had significantly lower CD103 + CD8 + T cell infiltration rate than patients without distant metastasis (P<0.01). There was no significant correlation between the infiltration of CD103 + CD8 + T cells and other clinicopathological features (P>0.05). Kaplan-Meier survival analysis showed that the overall survival (OS) of patients with high CD103 + CD8 + T cell infiltration was significantly longer than that of the patients with low infiltration (54.42% vs 25.00%, P<0.05). Multivariate Cox model analysis indicated that pathological grade (P<0.01) and high CD103 + CD8 + T cell infiltration (P<0.05) were independent prognostic factors for CRC. Conclusion：CD103+CD8+T cell infiltration in CRC is associated with patients’prognosis, suggesting that CD103 + CD8 + T cell plays an important role in the initiation and development of CRC.

AB - Objective: To investigate the degree and distribution of tissue-resident CD8 + T cell (CD103 + CD8 + T cells) infiltration in colorectal cancer (CRC) tissues, and to analyze its relationship to patients’clinicopathological features and prognosis. Methods: Tissue chips of 88 cases of colon cancer tissues (No.HColA180Su14) and 77 cases of rectal cancer tissues (No. HRec-Ade180Sur-03) were obtained from Shanghai Outdo Biotech Co., Ltd. Immunofluorescence staining was performed to examine the infiltration pattern and degree of CD103 + CD8 + T cells in the collected CRC tissues and their para-cancerous tissues. Wilcoxon rank test was used to compare CD103 + CD8 + T cell infiltration degree in CRC tissues and the para-cancerous tissues. Chi-square test was used to analyze the relationship between CD103 + CD8 + T cell infiltration in CRC and patients’clinicopathological features. Kaplan-Meier survival analysis was conducted to explore the correlation between CD103 + CD8 + T cell infiltration and patients’prognosis. Cox model was applied to analyze the correlation between different clinical parameters and patients’prognosis. Results: CD103 + CD8 + T cell infiltration presented no significant differences between CRC and para-cancer tissues (P>0.05). Patients with distant metastasis had significantly lower CD103 + CD8 + T cell infiltration rate than patients without distant metastasis (P<0.01). There was no significant correlation between the infiltration of CD103 + CD8 + T cells and other clinicopathological features (P>0.05). Kaplan-Meier survival analysis showed that the overall survival (OS) of patients with high CD103 + CD8 + T cell infiltration was significantly longer than that of the patients with low infiltration (54.42% vs 25.00%, P<0.05). Multivariate Cox model analysis indicated that pathological grade (P<0.01) and high CD103 + CD8 + T cell infiltration (P<0.05) were independent prognostic factors for CRC. Conclusion：CD103+CD8+T cell infiltration in CRC is associated with patients’prognosis, suggesting that CD103 + CD8 + T cell plays an important role in the initiation and development of CRC.

KW - CD103 CD8 T cell

KW - Colorectal cancer

KW - Prognosis

KW - Tissue microarray

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Lu H, Wang Y, Chen L, Jiang J, Lu B. Distribution of CD103 ⁺ CD8 ⁺ T cell infiltration in colorectal cancer tissues and its clinical significance Chinese Journal of Cancer Biotherapy. 2019 Jan;26(1):50-57. 1007-385X(2019)01-0050-08. doi: 10.3872/j.issn.1007-385X.2019.01.009