Evaluation of fluconazole resistance mechanisms in Candida albicans clinical isolates from HIV-infected patients in Brazil

Gustavo H. Goldman; Márcia Eliana Da Silva Ferreira; Everaldo Dos Reis Marques; Marcela Savoldi; David Perlin; Steven Park; Patricio Christian Godoy Martinez; Maria Helena S. Goldman; Arnaldo L. Colombo

doi:10.1016/j.diagmicrobio.2004.04.009

Evaluation of fluconazole resistance mechanisms in Candida albicans clinical isolates from HIV-infected patients in Brazil

Gustavo H. Goldman
, Márcia Eliana Da Silva Ferreira
, Everaldo Dos Reis Marques
, Marcela Savoldi
, David Perlin
, Steven Park
, Patricio Christian Godoy Martinez
, Maria Helena S. Goldman
, Arnaldo L. Colombo

Center for Discovery and Innovation

Universidade de São Paulo
Universidade Federal de São Paulo

Research output: Contribution to journal › Article › peer-review

107 Scopus citations

Abstract

In this study, we describe resistance mechanisms in fluconazole-resistant isolates of C. albicans isolated from AIDS patients from nine Brazilian hospitals. These mechanisms include the presence of point mutations in the ERG11 gene and overexpression of ERG11, and several genes encoding efflux pumps, as measured by quantitative real-time reverse transcriptase polymerase chain reaction. Several fluconazole-resistant strains had multiple mechanisms of resistance. Four mutations previously described, Y132F, K143R, E266D, and V437I, were identified among the strains, whereas some isolates contained more than one mutation. Fourteen novel mutations were identified. Interestingly, all Brazilian fluconazole-resistant isolates showed homozygosity at mating-type loci (MTL) associated with fluconazole resistance. This is the first comprehensive assessment at molecular level of mechanisms of fluconazole resistance in C. albicans isolates from South America.

Original language	English
Pages (from-to)	25-32
Number of pages	8
Journal	Diagnostic Microbiology and Infectious Disease
Volume	50
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2004.04.009
State	Published - Sep 2004

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10.1016/j.diagmicrobio.2004.04.009

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Goldman, G. H., Da Silva Ferreira, M. E., Dos Reis Marques, E., Savoldi, M., Perlin, D., Park, S., Godoy Martinez, P. C., Goldman, M. H. S., & Colombo, A. L. (2004). Evaluation of fluconazole resistance mechanisms in Candida albicans clinical isolates from HIV-infected patients in Brazil. Diagnostic Microbiology and Infectious Disease, 50(1), 25-32. https://doi.org/10.1016/j.diagmicrobio.2004.04.009

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title = "Evaluation of fluconazole resistance mechanisms in Candida albicans clinical isolates from HIV-infected patients in Brazil",

abstract = "In this study, we describe resistance mechanisms in fluconazole-resistant isolates of C. albicans isolated from AIDS patients from nine Brazilian hospitals. These mechanisms include the presence of point mutations in the ERG11 gene and overexpression of ERG11, and several genes encoding efflux pumps, as measured by quantitative real-time reverse transcriptase polymerase chain reaction. Several fluconazole-resistant strains had multiple mechanisms of resistance. Four mutations previously described, Y132F, K143R, E266D, and V437I, were identified among the strains, whereas some isolates contained more than one mutation. Fourteen novel mutations were identified. Interestingly, all Brazilian fluconazole-resistant isolates showed homozygosity at mating-type loci (MTL) associated with fluconazole resistance. This is the first comprehensive assessment at molecular level of mechanisms of fluconazole resistance in C. albicans isolates from South America.",

author = "Goldman, \{Gustavo H.\} and \{Da Silva Ferreira\}, \{M{\'a}rcia Eliana\} and \{Dos Reis Marques\}, Everaldo and Marcela Savoldi and David Perlin and Steven Park and \{Godoy Martinez\}, \{Patricio Christian\} and Goldman, \{Maria Helena S.\} and Colombo, \{Arnaldo L.\}",

year = "2004",

month = sep,

doi = "10.1016/j.diagmicrobio.2004.04.009",

language = "English",

volume = "50",

pages = "25--32",

journal = "Diagnostic Microbiology and Infectious Disease",

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publisher = "Elsevier Inc.",

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}

Goldman, GH, Da Silva Ferreira, ME, Dos Reis Marques, E, Savoldi, M, Perlin, D , Park, S, Godoy Martinez, PC, Goldman, MHS & Colombo, AL 2004, 'Evaluation of fluconazole resistance mechanisms in Candida albicans clinical isolates from HIV-infected patients in Brazil', Diagnostic Microbiology and Infectious Disease, vol. 50, no. 1, pp. 25-32. https://doi.org/10.1016/j.diagmicrobio.2004.04.009

Evaluation of fluconazole resistance mechanisms in Candida albicans clinical isolates from HIV-infected patients in Brazil. / Goldman, Gustavo H.; Da Silva Ferreira, Márcia Eliana; Dos Reis Marques, Everaldo et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 50, No. 1, 09.2004, p. 25-32.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Evaluation of fluconazole resistance mechanisms in Candida albicans clinical isolates from HIV-infected patients in Brazil

AU - Goldman, Gustavo H.

AU - Da Silva Ferreira, Márcia Eliana

AU - Dos Reis Marques, Everaldo

AU - Savoldi, Marcela

AU - Perlin, David

AU - Park, Steven

AU - Godoy Martinez, Patricio Christian

AU - Goldman, Maria Helena S.

AU - Colombo, Arnaldo L.

PY - 2004/9

Y1 - 2004/9

N2 - In this study, we describe resistance mechanisms in fluconazole-resistant isolates of C. albicans isolated from AIDS patients from nine Brazilian hospitals. These mechanisms include the presence of point mutations in the ERG11 gene and overexpression of ERG11, and several genes encoding efflux pumps, as measured by quantitative real-time reverse transcriptase polymerase chain reaction. Several fluconazole-resistant strains had multiple mechanisms of resistance. Four mutations previously described, Y132F, K143R, E266D, and V437I, were identified among the strains, whereas some isolates contained more than one mutation. Fourteen novel mutations were identified. Interestingly, all Brazilian fluconazole-resistant isolates showed homozygosity at mating-type loci (MTL) associated with fluconazole resistance. This is the first comprehensive assessment at molecular level of mechanisms of fluconazole resistance in C. albicans isolates from South America.

AB - In this study, we describe resistance mechanisms in fluconazole-resistant isolates of C. albicans isolated from AIDS patients from nine Brazilian hospitals. These mechanisms include the presence of point mutations in the ERG11 gene and overexpression of ERG11, and several genes encoding efflux pumps, as measured by quantitative real-time reverse transcriptase polymerase chain reaction. Several fluconazole-resistant strains had multiple mechanisms of resistance. Four mutations previously described, Y132F, K143R, E266D, and V437I, were identified among the strains, whereas some isolates contained more than one mutation. Fourteen novel mutations were identified. Interestingly, all Brazilian fluconazole-resistant isolates showed homozygosity at mating-type loci (MTL) associated with fluconazole resistance. This is the first comprehensive assessment at molecular level of mechanisms of fluconazole resistance in C. albicans isolates from South America.

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M3 - Article

C2 - 15380275

AN - SCOPUS:5444239537

SN - 0732-8893

VL - 50

SP - 25

EP - 32

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 1

ER -

Evaluation of fluconazole resistance mechanisms in Candida albicans clinical isolates from HIV-infected patients in Brazil

Abstract

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