Evaluation of murine renal phagocyte-fungal interactions using intravital confocal microscopy and flow cytometry

Jigar V. Desai; Michail S. Lionakis

doi:10.1016/j.xpro.2023.102781

Evaluation of murine renal phagocyte-fungal interactions using intravital confocal microscopy and flow cytometry

Jigar V. Desai
, Michail S. Lionakis

Center for Discovery and Innovation

National Institutes of Health
Lead contact

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Myeloid phagocytes are essential for antifungal host defense during systemic candidiasis. Here, we present a protocol for assessing phagocyte-fungal interactions in vivo in the kidney, the primary target organ of the murine systemic candidiasis model. We describe steps for intravital confocal microscopy and flow cytometry. We also detail a kidney tissue dissociation procedure to obtain highly pure functional phagocytes for utilization in downstream ex vivo fungal uptake and killing assays.

Original language	English
Article number	102781
Journal	STAR Protocols
Volume	5
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2023.102781
State	Published - 15 Mar 2024

Keywords

Immunology
Microbiology
Microscopy

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10.1016/j.xpro.2023.102781

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title = "Evaluation of murine renal phagocyte-fungal interactions using intravital confocal microscopy and flow cytometry",

abstract = "Myeloid phagocytes are essential for antifungal host defense during systemic candidiasis. Here, we present a protocol for assessing phagocyte-fungal interactions in vivo in the kidney, the primary target organ of the murine systemic candidiasis model. We describe steps for intravital confocal microscopy and flow cytometry. We also detail a kidney tissue dissociation procedure to obtain highly pure functional phagocytes for utilization in downstream ex vivo fungal uptake and killing assays.",

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AU - Lionakis, Michail S.

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N2 - Myeloid phagocytes are essential for antifungal host defense during systemic candidiasis. Here, we present a protocol for assessing phagocyte-fungal interactions in vivo in the kidney, the primary target organ of the murine systemic candidiasis model. We describe steps for intravital confocal microscopy and flow cytometry. We also detail a kidney tissue dissociation procedure to obtain highly pure functional phagocytes for utilization in downstream ex vivo fungal uptake and killing assays.

AB - Myeloid phagocytes are essential for antifungal host defense during systemic candidiasis. Here, we present a protocol for assessing phagocyte-fungal interactions in vivo in the kidney, the primary target organ of the murine systemic candidiasis model. We describe steps for intravital confocal microscopy and flow cytometry. We also detail a kidney tissue dissociation procedure to obtain highly pure functional phagocytes for utilization in downstream ex vivo fungal uptake and killing assays.

KW - Immunology

KW - Microbiology

KW - Microscopy

UR - https://www.scopus.com/pages/publications/85188480475

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DO - 10.1016/j.xpro.2023.102781

M3 - Article

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AN - SCOPUS:85188480475

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JO - STAR Protocols

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ER -

Evaluation of murine renal phagocyte-fungal interactions using intravital confocal microscopy and flow cytometry

Abstract

Keywords

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