GA binding protein regulates interleukin 7 receptor α-chain gene expression in T cells

Hai Hui Xue; Julie Bollenbacher; Valentina Rovella; Radhika Tripuraneni; Yu Bin Du; Cheng Yu Liu; Ann Williams; J. Philip McCoy; Warren J. Leonard

doi:10.1038/ni1117

GA binding protein regulates interleukin 7 receptor α-chain gene expression in T cells

Hai Hui Xue
, Julie Bollenbacher
, Valentina Rovella
, Radhika Tripuraneni
, Yu Bin Du
, Cheng Yu Liu
, Ann Williams
, J. Philip McCoy
, Warren J. Leonard

Center for Discovery and Innovation

National Institutes of Health
University of Modena and Reggio Emilia
Beth Israel Deaconess Medical Center

Research output: Contribution to journal › Article › peer-review

132 Scopus citations

Abstract

The interleukin 7 receptor α-chain (IL-7Rα) is essential for T cell development in both humans and mice and for B cell development in mice. Whereas the transcription factor PU.1 regulates IL-7Rα expression in mouse pro-B cells via a GGAA motif, we demonstrate here that GA binding protein (GABP) bound to this site and was essential in the regulation of IL-7Rα expression in T cells, where PU.1 is not expressed. Moreover, IL-7Rα expression was diminished substantially in thymocytes but was normal on B220⁺ fetal liver cells from mouse embryos with diminished expression of GABPα. Thus, GABP is essential for the regulation of IL-7Rα expression in T cells, and the differential regulation of IL-7Rα in distinct lymphoid lineages is achieved at least in part by differential recruitment of factors to the same GGAA motif.

Original language	English
Pages (from-to)	1036-1044
Number of pages	9
Journal	Nature Immunology
Volume	5
Issue number	10
DOIs	https://doi.org/10.1038/ni1117
State	Published - Oct 2004

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title = "GA binding protein regulates interleukin 7 receptor α-chain gene expression in T cells",

abstract = "The interleukin 7 receptor α-chain (IL-7Rα) is essential for T cell development in both humans and mice and for B cell development in mice. Whereas the transcription factor PU.1 regulates IL-7Rα expression in mouse pro-B cells via a GGAA motif, we demonstrate here that GA binding protein (GABP) bound to this site and was essential in the regulation of IL-7Rα expression in T cells, where PU.1 is not expressed. Moreover, IL-7Rα expression was diminished substantially in thymocytes but was normal on B220+ fetal liver cells from mouse embryos with diminished expression of GABPα. Thus, GABP is essential for the regulation of IL-7Rα expression in T cells, and the differential regulation of IL-7Rα in distinct lymphoid lineages is achieved at least in part by differential recruitment of factors to the same GGAA motif.",

author = "Xue, \{Hai Hui\} and Julie Bollenbacher and Valentina Rovella and Radhika Tripuraneni and Du, \{Yu Bin\} and Liu, \{Cheng Yu\} and Ann Williams and McCoy, \{J. Philip\} and Leonard, \{Warren J.\}",

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AU - Xue, Hai Hui

AU - Bollenbacher, Julie

AU - Rovella, Valentina

AU - Tripuraneni, Radhika

AU - Du, Yu Bin

AU - Liu, Cheng Yu

AU - Williams, Ann

AU - McCoy, J. Philip

AU - Leonard, Warren J.

PY - 2004/10

Y1 - 2004/10

N2 - The interleukin 7 receptor α-chain (IL-7Rα) is essential for T cell development in both humans and mice and for B cell development in mice. Whereas the transcription factor PU.1 regulates IL-7Rα expression in mouse pro-B cells via a GGAA motif, we demonstrate here that GA binding protein (GABP) bound to this site and was essential in the regulation of IL-7Rα expression in T cells, where PU.1 is not expressed. Moreover, IL-7Rα expression was diminished substantially in thymocytes but was normal on B220+ fetal liver cells from mouse embryos with diminished expression of GABPα. Thus, GABP is essential for the regulation of IL-7Rα expression in T cells, and the differential regulation of IL-7Rα in distinct lymphoid lineages is achieved at least in part by differential recruitment of factors to the same GGAA motif.

AB - The interleukin 7 receptor α-chain (IL-7Rα) is essential for T cell development in both humans and mice and for B cell development in mice. Whereas the transcription factor PU.1 regulates IL-7Rα expression in mouse pro-B cells via a GGAA motif, we demonstrate here that GA binding protein (GABP) bound to this site and was essential in the regulation of IL-7Rα expression in T cells, where PU.1 is not expressed. Moreover, IL-7Rα expression was diminished substantially in thymocytes but was normal on B220+ fetal liver cells from mouse embryos with diminished expression of GABPα. Thus, GABP is essential for the regulation of IL-7Rα expression in T cells, and the differential regulation of IL-7Rα in distinct lymphoid lineages is achieved at least in part by differential recruitment of factors to the same GGAA motif.

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M3 - Article

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VL - 5

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JO - Nature Immunology

JF - Nature Immunology

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ER -

GA binding protein regulates interleukin 7 receptor α-chain gene expression in T cells

Abstract

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