Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis

V. Dartois; J. Y. Coppee; C. Colson; A. Baulard

doi:10.1128/aem.60.5.1670-1673.1994

Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis

V. Dartois
, J. Y. Coppee
, C. Colson
, A. Baulard

Center for Discovery and Innovation

Scripps Research Institute

Research output: Contribution to journal › Comment/debate

61 Scopus citations

Abstract

The previously cloned Bacillus subtilis lipase gene (lip) was mapped on the chromosome and used in the construction of a B. subtilis derivative totally devoid of any lip sequence. Homologous overexpression was performed in this strain by subcloning the lip open reading frame on a multicopy plasmid under the control of a strong gram-positive promoter. A 100-fold overproducing strain was obtained, which should facilitate purification of the secreted protein. Furthermore, the Δlip strain BCL1050 constitutes an ideal host for the cloning of heterologous lipase genes.

Original language	English
Pages (from-to)	1670-1673
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	60
Issue number	5
DOIs	https://doi.org/10.1128/aem.60.5.1670-1673.1994
State	Published - 1994

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10.1128/aem.60.5.1670-1673.1994

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title = "Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis",

abstract = "The previously cloned Bacillus subtilis lipase gene (lip) was mapped on the chromosome and used in the construction of a B. subtilis derivative totally devoid of any lip sequence. Homologous overexpression was performed in this strain by subcloning the lip open reading frame on a multicopy plasmid under the control of a strong gram-positive promoter. A 100-fold overproducing strain was obtained, which should facilitate purification of the secreted protein. Furthermore, the Δlip strain BCL1050 constitutes an ideal host for the cloning of heterologous lipase genes.",

author = "V. Dartois and Coppee, \{J. Y.\} and C. Colson and A. Baulard",

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doi = "10.1128/aem.60.5.1670-1673.1994",

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T1 - Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis

AU - Dartois, V.

AU - Coppee, J. Y.

AU - Colson, C.

AU - Baulard, A.

PY - 1994

Y1 - 1994

N2 - The previously cloned Bacillus subtilis lipase gene (lip) was mapped on the chromosome and used in the construction of a B. subtilis derivative totally devoid of any lip sequence. Homologous overexpression was performed in this strain by subcloning the lip open reading frame on a multicopy plasmid under the control of a strong gram-positive promoter. A 100-fold overproducing strain was obtained, which should facilitate purification of the secreted protein. Furthermore, the Δlip strain BCL1050 constitutes an ideal host for the cloning of heterologous lipase genes.

AB - The previously cloned Bacillus subtilis lipase gene (lip) was mapped on the chromosome and used in the construction of a B. subtilis derivative totally devoid of any lip sequence. Homologous overexpression was performed in this strain by subcloning the lip open reading frame on a multicopy plasmid under the control of a strong gram-positive promoter. A 100-fold overproducing strain was obtained, which should facilitate purification of the secreted protein. Furthermore, the Δlip strain BCL1050 constitutes an ideal host for the cloning of heterologous lipase genes.

UR - https://www.scopus.com/pages/publications/0028197268

U2 - 10.1128/aem.60.5.1670-1673.1994

DO - 10.1128/aem.60.5.1670-1673.1994

M3 - Comment/debate

C2 - 8017946

AN - SCOPUS:0028197268

SN - 0099-2240

VL - 60

SP - 1670

EP - 1673

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 5

ER -

Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis

Abstract

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