In vitro analysis of peroxisomal protein import.

Stanley R. Terlecky

doi:10.1002/0471143030.cb1115s14

In vitro analysis of peroxisomal protein import.

Stanley R. Terlecky

School of Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

This unit describes a quantitative in vitro assay for peroxisomal protein import that accurately reconstitutes established properties of the pathway. The cell-free system is ELISA based and employs semi-permeabilized human cells with a biotinylated peroxisomal targeting signal-containing substrate.

Original language	English
Pages (from-to)	Unit 11.15
Journal	Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]
Volume	Chapter 11
DOIs	https://doi.org/10.1002/0471143030.cb1115s14
State	Published - May 2002

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title = "In vitro analysis of peroxisomal protein import.",

abstract = "This unit describes a quantitative in vitro assay for peroxisomal protein import that accurately reconstitutes established properties of the pathway. The cell-free system is ELISA based and employs semi-permeabilized human cells with a biotinylated peroxisomal targeting signal-containing substrate.",

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language = "English",

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AB - This unit describes a quantitative in vitro assay for peroxisomal protein import that accurately reconstitutes established properties of the pathway. The cell-free system is ELISA based and employs semi-permeabilized human cells with a biotinylated peroxisomal targeting signal-containing substrate.

UR - https://www.scopus.com/pages/publications/39749118654

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DO - 10.1002/0471143030.cb1115s14

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C2 - 18228397

AN - SCOPUS:39749118654

SN - 1934-2616

VL - Chapter 11

SP - Unit 11.15

JO - Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]

JF - Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]

ER -

In vitro analysis of peroxisomal protein import.

Abstract

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