@inbook{26726928bc864547a9989bee4ea5a08d,
title = "Isolation and propagation of glioma stem cells from acutely resected tumors",
abstract = "Gliomas are characterized by striking intratumoral cellular heterogeneity. A consistent method to isolate undifferentiated GSC fraction from clinical specimens provides an opportunity to establish in vitro models that more closely mirror the in vivo state compared to traditional serum containing culture methods. Here we describe techniques involved with isolation, identification, and expansion of human malignant gliomaderived stem cells using a serum-free growth condition.",
keywords = "GBM, Glioblastoma multiforme, Glioma stem cell, N2 medium, Neural stem cell, Serum-free cell culture",
author = "Jinkyu Jung and Gilbert, \{Mark R.\} and Park, \{Deric M.\}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
doi = "10.1007/7651\_2016\_342",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "361--369",
booktitle = "Methods in Molecular Biology",
}