Molecular genetic characterisation of whiB3, a mycobacterial homologue of a Streptomyces sporulation factor

Bernd Hutter; Thomas Dick

doi:10.1016/S0923-2508(99)80055-2

Molecular genetic characterisation of whiB3, a mycobacterial homologue of a Streptomyces sporulation factor

Bernd Hutter
, Thomas Dick

Center for Discovery and Innovation

Agency for Science, Technology and Research, Singapore

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

WhiB is an essential sporulation factor in Streptomyces coelicolor. We report here the molecular genetic characterisation of whiB3, a whiB-like gene in the nonspore-forming Mycobacterium smegmatis mc²155. M. smegmatis whiB3 encodes a 96-amino-acid protein with 81% similarity to its M. tuberculosis counterpart identified in the genome project, and 35% similarity to S. coelicolor WhiB. In both mycobacteria, whiB3 is flanked by the same upstream gene, Rv3415c, and appears to be monocistronic, promoter probe analyses suggest that the whiB3 gene is expressed constitutively. Disruption of whiB3 did not affect growth or the dormancy response of M. smegmatis.

Original language	English
Pages (from-to)	295-301
Number of pages	7
Journal	Research in Microbiology
Volume	150
Issue number	5
DOIs	https://doi.org/10.1016/S0923-2508(99)80055-2
State	Published - Jun 1999

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Bacterial growth
Gene disruption
Gene whiB
Mycobacterium smegmatis
Sporulation
Streptomyces coelicolor

Access to Document

10.1016/S0923-2508(99)80055-2

Cite this

@article{995a414134b841f4a9edaeeaa6c6ae4c,

title = "Molecular genetic characterisation of whiB3, a mycobacterial homologue of a Streptomyces sporulation factor",

abstract = "WhiB is an essential sporulation factor in Streptomyces coelicolor. We report here the molecular genetic characterisation of whiB3, a whiB-like gene in the nonspore-forming Mycobacterium smegmatis mc2155. M. smegmatis whiB3 encodes a 96-amino-acid protein with 81\% similarity to its M. tuberculosis counterpart identified in the genome project, and 35\% similarity to S. coelicolor WhiB. In both mycobacteria, whiB3 is flanked by the same upstream gene, Rv3415c, and appears to be monocistronic, promoter probe analyses suggest that the whiB3 gene is expressed constitutively. Disruption of whiB3 did not affect growth or the dormancy response of M. smegmatis.",

keywords = "Bacterial growth, Gene disruption, Gene whiB, Mycobacterium smegmatis, Sporulation, Streptomyces coelicolor",

author = "Bernd Hutter and Thomas Dick",

year = "1999",

month = jun,

doi = "10.1016/S0923-2508(99)80055-2",

language = "English",

volume = "150",

pages = "295--301",

journal = "Research in Microbiology",

issn = "0923-2508",

publisher = "Elsevier Masson s.r.l.",

number = "5",

}

TY - JOUR

T1 - Molecular genetic characterisation of whiB3, a mycobacterial homologue of a Streptomyces sporulation factor

AU - Hutter, Bernd

AU - Dick, Thomas

PY - 1999/6

Y1 - 1999/6

N2 - WhiB is an essential sporulation factor in Streptomyces coelicolor. We report here the molecular genetic characterisation of whiB3, a whiB-like gene in the nonspore-forming Mycobacterium smegmatis mc2155. M. smegmatis whiB3 encodes a 96-amino-acid protein with 81% similarity to its M. tuberculosis counterpart identified in the genome project, and 35% similarity to S. coelicolor WhiB. In both mycobacteria, whiB3 is flanked by the same upstream gene, Rv3415c, and appears to be monocistronic, promoter probe analyses suggest that the whiB3 gene is expressed constitutively. Disruption of whiB3 did not affect growth or the dormancy response of M. smegmatis.

AB - WhiB is an essential sporulation factor in Streptomyces coelicolor. We report here the molecular genetic characterisation of whiB3, a whiB-like gene in the nonspore-forming Mycobacterium smegmatis mc2155. M. smegmatis whiB3 encodes a 96-amino-acid protein with 81% similarity to its M. tuberculosis counterpart identified in the genome project, and 35% similarity to S. coelicolor WhiB. In both mycobacteria, whiB3 is flanked by the same upstream gene, Rv3415c, and appears to be monocistronic, promoter probe analyses suggest that the whiB3 gene is expressed constitutively. Disruption of whiB3 did not affect growth or the dormancy response of M. smegmatis.

KW - Bacterial growth

KW - Gene disruption

KW - Gene whiB

KW - Mycobacterium smegmatis

KW - Sporulation

KW - Streptomyces coelicolor

UR - https://www.scopus.com/pages/publications/0032725878

U2 - 10.1016/S0923-2508(99)80055-2

DO - 10.1016/S0923-2508(99)80055-2

M3 - Article

C2 - 10422690

AN - SCOPUS:0032725878

SN - 0923-2508

VL - 150

SP - 295

EP - 301

JO - Research in Microbiology

JF - Research in Microbiology

IS - 5

ER -

Molecular genetic characterisation of whiB3, a mycobacterial homologue of a Streptomyces sporulation factor

Abstract

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this