Mycobacterium tuberculosis in Ontario, Canada: Insights from IS6110 restriction fragment length polymorphism and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping

David C. Alexander; Jennifer L. Guthrie; Daria Pyskir; Anne Maki; Natalia Kurepina; Barry N. Kreiswirth; Pamela Chedore; Steven J. Drews; Frances Jamieson

doi:10.1128/JCM.01946-08

Mycobacterium tuberculosis in Ontario, Canada: Insights from IS6110 restriction fragment length polymorphism and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping

David C. Alexander
, Jennifer L. Guthrie
, Daria Pyskir
, Anne Maki
, Natalia Kurepina
, Barry N. Kreiswirth
, Pamela Chedore
, Steven J. Drews
, Frances Jamieson

Public Health Ontario

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

A collection of 1,308 clinical Mycobacterium tuberculosis isolates from Ontario, Canada, was genotyped by IS6110 restriction fragment length polymorphism (RFLP) and mycobacterial interspersed repetitive-unit-variable- number tandem-repeat (MIRU-VNTR) analysis. RFLP or >12 MIRU-VNTR loci were necessary for resolution of Indo-Oceanic strains. The low clustering rate and high strain diversity indicate that, in Ontario, most tuberculosis results from reactivation of latent infections.

Original language	English
Pages (from-to)	2651-2654
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	47
Issue number	8
DOIs	https://doi.org/10.1128/JCM.01946-08
State	Published - Aug 2009

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SDG 3 Good Health and Well-being

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10.1128/JCM.01946-08

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Alexander, D. C., Guthrie, J. L., Pyskir, D., Maki, A., Kurepina, N., Kreiswirth, B. N., Chedore, P., Drews, S. J., & Jamieson, F. (2009). Mycobacterium tuberculosis in Ontario, Canada: Insights from IS6110 restriction fragment length polymorphism and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping. Journal of Clinical Microbiology, 47(8), 2651-2654. https://doi.org/10.1128/JCM.01946-08

@article{2528ff9e99834e83bc6c85c5ba8193ed,

title = "Mycobacterium tuberculosis in Ontario, Canada: Insights from IS6110 restriction fragment length polymorphism and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping",

abstract = "A collection of 1,308 clinical Mycobacterium tuberculosis isolates from Ontario, Canada, was genotyped by IS6110 restriction fragment length polymorphism (RFLP) and mycobacterial interspersed repetitive-unit-variable- number tandem-repeat (MIRU-VNTR) analysis. RFLP or >12 MIRU-VNTR loci were necessary for resolution of Indo-Oceanic strains. The low clustering rate and high strain diversity indicate that, in Ontario, most tuberculosis results from reactivation of latent infections.",

author = "Alexander, \{David C.\} and Guthrie, \{Jennifer L.\} and Daria Pyskir and Anne Maki and Natalia Kurepina and Kreiswirth, \{Barry N.\} and Pamela Chedore and Drews, \{Steven J.\} and Frances Jamieson",

year = "2009",

month = aug,

doi = "10.1128/JCM.01946-08",

language = "English",

volume = "47",

pages = "2651--2654",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "8",

}

Alexander, DC, Guthrie, JL, Pyskir, D, Maki, A, Kurepina, N , Kreiswirth, BN, Chedore, P, Drews, SJ & Jamieson, F 2009, 'Mycobacterium tuberculosis in Ontario, Canada: Insights from IS6110 restriction fragment length polymorphism and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping', Journal of Clinical Microbiology, vol. 47, no. 8, pp. 2651-2654. https://doi.org/10.1128/JCM.01946-08

Mycobacterium tuberculosis in Ontario, Canada: Insights from IS6110 restriction fragment length polymorphism and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping. / Alexander, David C.; Guthrie, Jennifer L.; Pyskir, Daria et al.
In: Journal of Clinical Microbiology, Vol. 47, No. 8, 08.2009, p. 2651-2654.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Mycobacterium tuberculosis in Ontario, Canada

T2 - Insights from IS6110 restriction fragment length polymorphism and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping

AU - Alexander, David C.

AU - Guthrie, Jennifer L.

AU - Pyskir, Daria

AU - Maki, Anne

AU - Kurepina, Natalia

AU - Kreiswirth, Barry N.

AU - Chedore, Pamela

AU - Drews, Steven J.

AU - Jamieson, Frances

PY - 2009/8

Y1 - 2009/8

N2 - A collection of 1,308 clinical Mycobacterium tuberculosis isolates from Ontario, Canada, was genotyped by IS6110 restriction fragment length polymorphism (RFLP) and mycobacterial interspersed repetitive-unit-variable- number tandem-repeat (MIRU-VNTR) analysis. RFLP or >12 MIRU-VNTR loci were necessary for resolution of Indo-Oceanic strains. The low clustering rate and high strain diversity indicate that, in Ontario, most tuberculosis results from reactivation of latent infections.

AB - A collection of 1,308 clinical Mycobacterium tuberculosis isolates from Ontario, Canada, was genotyped by IS6110 restriction fragment length polymorphism (RFLP) and mycobacterial interspersed repetitive-unit-variable- number tandem-repeat (MIRU-VNTR) analysis. RFLP or >12 MIRU-VNTR loci were necessary for resolution of Indo-Oceanic strains. The low clustering rate and high strain diversity indicate that, in Ontario, most tuberculosis results from reactivation of latent infections.

UR - https://www.scopus.com/pages/publications/68549133198

U2 - 10.1128/JCM.01946-08

DO - 10.1128/JCM.01946-08

M3 - Article

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AN - SCOPUS:68549133198

SN - 0095-1137

VL - 47

SP - 2651

EP - 2654

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 8

ER -

Mycobacterium tuberculosis in Ontario, Canada: Insights from IS6110 restriction fragment length polymorphism and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping

Abstract

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