Promoter variation and gene expression of mcr-1-harboring plasmids in clinical isolates of Escherichia coli and klebsiella pneumoniae from a Chinese Hospital

Bin Huang; Yuting He; Xingyan Ma; Renxin Cai; Jianming Zeng; Yang Lu; Weizheng Zhang; Kai Lan; E. Sunmei; Yi Wei Tang; Barry N. Kreiswirth; Cha Chen; Liang Chen

doi:10.1128/AAC.00018-18

Promoter variation and gene expression of mcr-1-harboring plasmids in clinical isolates of Escherichia coli and klebsiella pneumoniae from a Chinese Hospital

Bin Huang
, Yuting He
, Xingyan Ma
, Renxin Cai
, Jianming Zeng
, Yang Lu
, Weizheng Zhang
, Kai Lan
, E. Sunmei
, Yi Wei Tang
, Barry N. Kreiswirth
, Cha Chen
, Liang Chen

First Affiliated Hospital of Sun Yat-sen University
Guangdong Provincial Hospital of Traditional Chinese Medicine
Memorial Sloan-Kettering Cancer Center
RBHS-NEW JERSEY MEDICAL SCHOOL

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Next-generation sequencing of 6 mcr-1-harboring Escherichia coli and Klebsiella pneumoniae isolates collected from a tertiary care hospital in China revealed significant sequence variations in the regions flanking the mcr-1 gene. While sequence variations significantly affected the expression and promoter activity of mcr-1, the mcr-1 gene expression levels did not correlate with the in vitro colistin resistance levels, which warrants further in-depth investigations.

Original language	English
Article number	e00018
Journal	Antimicrobial Agents and Chemotherapy
Volume	62
Issue number	5
DOIs	https://doi.org/10.1128/AAC.00018-18
State	Published - May 2018

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Colistin
Gene expression
Genomics
Plasmids
Promoter

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10.1128/AAC.00018-18

Cite this

Huang, B., He, Y., Ma, X., Cai, R., Zeng, J., Lu, Y., Zhang, W., Lan, K., Sunmei, E., Tang, Y. W., Kreiswirth, B. N., Chen, C., & Chen, L. (2018). Promoter variation and gene expression of mcr-1-harboring plasmids in clinical isolates of Escherichia coli and klebsiella pneumoniae from a Chinese Hospital. Antimicrobial Agents and Chemotherapy, 62(5), Article e00018. https://doi.org/10.1128/AAC.00018-18

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title = "Promoter variation and gene expression of mcr-1-harboring plasmids in clinical isolates of Escherichia coli and klebsiella pneumoniae from a Chinese Hospital",

abstract = "Next-generation sequencing of 6 mcr-1-harboring Escherichia coli and Klebsiella pneumoniae isolates collected from a tertiary care hospital in China revealed significant sequence variations in the regions flanking the mcr-1 gene. While sequence variations significantly affected the expression and promoter activity of mcr-1, the mcr-1 gene expression levels did not correlate with the in vitro colistin resistance levels, which warrants further in-depth investigations.",

keywords = "Colistin, Gene expression, Genomics, Plasmids, Promoter",

author = "Bin Huang and Yuting He and Xingyan Ma and Renxin Cai and Jianming Zeng and Yang Lu and Weizheng Zhang and Kai Lan and E. Sunmei and Tang, \{Yi Wei\} and Kreiswirth, \{Barry N.\} and Cha Chen and Liang Chen",

year = "2018",

month = may,

doi = "10.1128/AAC.00018-18",

language = "English",

volume = "62",

journal = "Antimicrobial Agents and Chemotherapy",

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Huang, B, He, Y, Ma, X, Cai, R, Zeng, J, Lu, Y, Zhang, W, Lan, K, Sunmei, E, Tang, YW, Kreiswirth, BN, Chen, C & Chen, L 2018, 'Promoter variation and gene expression of mcr-1-harboring plasmids in clinical isolates of Escherichia coli and klebsiella pneumoniae from a Chinese Hospital', Antimicrobial Agents and Chemotherapy, vol. 62, no. 5, e00018. https://doi.org/10.1128/AAC.00018-18

TY - JOUR

T1 - Promoter variation and gene expression of mcr-1-harboring plasmids in clinical isolates of Escherichia coli and klebsiella pneumoniae from a Chinese Hospital

AU - Huang, Bin

AU - He, Yuting

AU - Ma, Xingyan

AU - Cai, Renxin

AU - Zeng, Jianming

AU - Lu, Yang

AU - Zhang, Weizheng

AU - Lan, Kai

AU - Sunmei, E.

AU - Tang, Yi Wei

AU - Kreiswirth, Barry N.

AU - Chen, Cha

AU - Chen, Liang

PY - 2018/5

Y1 - 2018/5

N2 - Next-generation sequencing of 6 mcr-1-harboring Escherichia coli and Klebsiella pneumoniae isolates collected from a tertiary care hospital in China revealed significant sequence variations in the regions flanking the mcr-1 gene. While sequence variations significantly affected the expression and promoter activity of mcr-1, the mcr-1 gene expression levels did not correlate with the in vitro colistin resistance levels, which warrants further in-depth investigations.

AB - Next-generation sequencing of 6 mcr-1-harboring Escherichia coli and Klebsiella pneumoniae isolates collected from a tertiary care hospital in China revealed significant sequence variations in the regions flanking the mcr-1 gene. While sequence variations significantly affected the expression and promoter activity of mcr-1, the mcr-1 gene expression levels did not correlate with the in vitro colistin resistance levels, which warrants further in-depth investigations.

KW - Colistin

KW - Gene expression

KW - Genomics

KW - Plasmids

KW - Promoter

UR - https://www.scopus.com/pages/publications/85046041299

U2 - 10.1128/AAC.00018-18

DO - 10.1128/AAC.00018-18

M3 - Article

C2 - 29463536

AN - SCOPUS:85046041299

SN - 0066-4804

VL - 62

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 5

M1 - e00018

ER -

Promoter variation and gene expression of mcr-1-harboring plasmids in clinical isolates of Escherichia coli and klebsiella pneumoniae from a Chinese Hospital

Abstract

UN SDGs

Keywords

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