Protective capacity of proteoliposomes from Mycobacterium bovis BCG in a mouse model of tuberculosis

Yanely Tirado; Alina Puig; Nadine Alvarez; Reinier Borrero; Alicia Aguilar; Frank Camacho; Fatima Reyes; Sonsire Fernández; José Luis Pérez; Dulce Mata Espinoza; Jorge Alberto Barrios Payán; María Elena Sarmiento; Mohd Nor Norazmi; Rogelio Hernández-Pando; Armando Acosta

doi:10.1080/21645515.2015.1011566

Protective capacity of proteoliposomes from Mycobacterium bovis BCG in a mouse model of tuberculosis

Yanely Tirado
, Alina Puig
, Nadine Alvarez
, Reinier Borrero
, Alicia Aguilar
, Frank Camacho
, Fatima Reyes
, Sonsire Fernández
, José Luis Pérez
, Dulce Mata Espinoza
, Jorge Alberto Barrios Payán
, María Elena Sarmiento
, Mohd Nor Norazmi
, Rogelio Hernández-Pando
, Armando Acosta

Center for Discovery and Innovation

Institute Finlay
Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran
Universiti Sains Malaysia

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Tuberculosis (TB) is one of the most important causes of mortality and morbidity due to infectious diseases. BCG, the vaccine in use, is not fully protective against TB. In a previous study, we have shown that proteoliposomes (outer membrane extracts), obtained from BCG (PLBCG) were able to induce humoral immune responses against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLBCG alone or as a booster with BCG, a murine model of progressive pulmonary TB was used. Animals immunized with PLBCG adjuvanted with alum (PLBCG-Al) showed similar protection to that conferred by BCG. The group immunized with PLBCG-Al as a booster to BCG gave superior protection than BCG as evidenced by a reduction of bacterial load in lungs 2 months after infection with Mtb. Animals immunized with BCG, PLBCG-Al and this formulation as a booster of BCG, showed a significant decrease of tissue damage (percentage of pneumonic area/lung) compared with non-immunized animals. These results demonstrate that immunization with PLBCG-Al alone or as a booster to BCG induce appropriate protection against challenge with Mtb in mice and support the future evaluation of PLBCG as a promising vaccine candidate against Mtb.

Original language	English
Pages (from-to)	657-661
Number of pages	5
Journal	Human Vaccines and Immunotherapeutics
Volume	11
Issue number	3
DOIs	https://doi.org/10.1080/21645515.2015.1011566
State	Published - 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

BCG
Prime-boost
Proteoliposomes
Tuberculosis
Vaccines

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10.1080/21645515.2015.1011566

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Tirado, Y., Puig, A., Alvarez, N., Borrero, R., Aguilar, A., Camacho, F., Reyes, F., Fernández, S., Pérez, J. L., Espinoza, D. M., Payán, J. A. B., Sarmiento, M. E., Norazmi, M. N., Hernández-Pando, R., & Acosta, A. (2015). Protective capacity of proteoliposomes from Mycobacterium bovis BCG in a mouse model of tuberculosis. Human Vaccines and Immunotherapeutics, 11(3), 657-661. https://doi.org/10.1080/21645515.2015.1011566

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title = "Protective capacity of proteoliposomes from Mycobacterium bovis BCG in a mouse model of tuberculosis",

abstract = "Tuberculosis (TB) is one of the most important causes of mortality and morbidity due to infectious diseases. BCG, the vaccine in use, is not fully protective against TB. In a previous study, we have shown that proteoliposomes (outer membrane extracts), obtained from BCG (PLBCG) were able to induce humoral immune responses against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLBCG alone or as a booster with BCG, a murine model of progressive pulmonary TB was used. Animals immunized with PLBCG adjuvanted with alum (PLBCG-Al) showed similar protection to that conferred by BCG. The group immunized with PLBCG-Al as a booster to BCG gave superior protection than BCG as evidenced by a reduction of bacterial load in lungs 2 months after infection with Mtb. Animals immunized with BCG, PLBCG-Al and this formulation as a booster of BCG, showed a significant decrease of tissue damage (percentage of pneumonic area/lung) compared with non-immunized animals. These results demonstrate that immunization with PLBCG-Al alone or as a booster to BCG induce appropriate protection against challenge with Mtb in mice and support the future evaluation of PLBCG as a promising vaccine candidate against Mtb.",

keywords = "BCG, Prime-boost, Proteoliposomes, Tuberculosis, Vaccines",

author = "Yanely Tirado and Alina Puig and Nadine Alvarez and Reinier Borrero and Alicia Aguilar and Frank Camacho and Fatima Reyes and Sonsire Fern{\'a}ndez and P{\'e}rez, \{Jos{\'e} Luis\} and Espinoza, \{Dulce Mata\} and Pay{\'a}n, \{Jorge Alberto Barrios\} and Sarmiento, \{Mar{\'i}a Elena\} and Norazmi, \{Mohd Nor\} and Rogelio Hern{\'a}ndez-Pando and Armando Acosta",

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doi = "10.1080/21645515.2015.1011566",

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Tirado, Y, Puig, A, Alvarez, N, Borrero, R, Aguilar, A, Camacho, F, Reyes, F, Fernández, S, Pérez, JL, Espinoza, DM, Payán, JAB, Sarmiento, ME, Norazmi, MN, Hernández-Pando, R & Acosta, A 2015, 'Protective capacity of proteoliposomes from Mycobacterium bovis BCG in a mouse model of tuberculosis', Human Vaccines and Immunotherapeutics, vol. 11, no. 3, pp. 657-661. https://doi.org/10.1080/21645515.2015.1011566

TY - JOUR

T1 - Protective capacity of proteoliposomes from Mycobacterium bovis BCG in a mouse model of tuberculosis

AU - Tirado, Yanely

AU - Puig, Alina

AU - Alvarez, Nadine

AU - Borrero, Reinier

AU - Aguilar, Alicia

AU - Camacho, Frank

AU - Reyes, Fatima

AU - Fernández, Sonsire

AU - Pérez, José Luis

AU - Espinoza, Dulce Mata

AU - Payán, Jorge Alberto Barrios

AU - Sarmiento, María Elena

AU - Norazmi, Mohd Nor

AU - Hernández-Pando, Rogelio

AU - Acosta, Armando

PY - 2015

Y1 - 2015

N2 - Tuberculosis (TB) is one of the most important causes of mortality and morbidity due to infectious diseases. BCG, the vaccine in use, is not fully protective against TB. In a previous study, we have shown that proteoliposomes (outer membrane extracts), obtained from BCG (PLBCG) were able to induce humoral immune responses against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLBCG alone or as a booster with BCG, a murine model of progressive pulmonary TB was used. Animals immunized with PLBCG adjuvanted with alum (PLBCG-Al) showed similar protection to that conferred by BCG. The group immunized with PLBCG-Al as a booster to BCG gave superior protection than BCG as evidenced by a reduction of bacterial load in lungs 2 months after infection with Mtb. Animals immunized with BCG, PLBCG-Al and this formulation as a booster of BCG, showed a significant decrease of tissue damage (percentage of pneumonic area/lung) compared with non-immunized animals. These results demonstrate that immunization with PLBCG-Al alone or as a booster to BCG induce appropriate protection against challenge with Mtb in mice and support the future evaluation of PLBCG as a promising vaccine candidate against Mtb.

AB - Tuberculosis (TB) is one of the most important causes of mortality and morbidity due to infectious diseases. BCG, the vaccine in use, is not fully protective against TB. In a previous study, we have shown that proteoliposomes (outer membrane extracts), obtained from BCG (PLBCG) were able to induce humoral immune responses against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLBCG alone or as a booster with BCG, a murine model of progressive pulmonary TB was used. Animals immunized with PLBCG adjuvanted with alum (PLBCG-Al) showed similar protection to that conferred by BCG. The group immunized with PLBCG-Al as a booster to BCG gave superior protection than BCG as evidenced by a reduction of bacterial load in lungs 2 months after infection with Mtb. Animals immunized with BCG, PLBCG-Al and this formulation as a booster of BCG, showed a significant decrease of tissue damage (percentage of pneumonic area/lung) compared with non-immunized animals. These results demonstrate that immunization with PLBCG-Al alone or as a booster to BCG induce appropriate protection against challenge with Mtb in mice and support the future evaluation of PLBCG as a promising vaccine candidate against Mtb.

KW - BCG

KW - Prime-boost

KW - Proteoliposomes

KW - Tuberculosis

KW - Vaccines

UR - https://www.scopus.com/pages/publications/84928690165

U2 - 10.1080/21645515.2015.1011566

DO - 10.1080/21645515.2015.1011566

M3 - Article

C2 - 25671612

AN - SCOPUS:84928690165

SN - 2164-5515

VL - 11

SP - 657

EP - 661

JO - Human Vaccines and Immunotherapeutics

JF - Human Vaccines and Immunotherapeutics

IS - 3

ER -

Protective capacity of proteoliposomes from Mycobacterium bovis BCG in a mouse model of tuberculosis

Abstract

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Keywords

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