Quick detection of FKS1 mutations responsible for clinical echinocandin resistance in Candida albicans

Catiana Dudiuk; Soledad Gamarra; Cristina Jimenez-Ortigosa; Florencia Leonardelli; Daiana Macedo; David S. Perlin; Guillermo Garcia-Effron

doi:10.1128/JCM.00398-15

Quick detection of FKS1 mutations responsible for clinical echinocandin resistance in Candida albicans

Catiana Dudiuk
, Soledad Gamarra
, Cristina Jimenez-Ortigosa
, Florencia Leonardelli
, Daiana Macedo
, David S. Perlin
, Guillermo Garcia-Effron

Center for Discovery and Innovation

Universidad Nacional del Litoral
Consejo Nacional de Investigaciones Científicas y Técnicas
Public Health Research Institute, New York

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

A rapid molecular-based assay for the detection of the Candida albicans FKS1 gene mutations responsible for resistance to echinocandin drugs was designed and evaluated. The assay consisted of a multiplexed PCR set of 5 tubes able to detect the most commonly described resistance mechanism, including FKS1 hot spot 1 and hot spot 2 mutations. The performance and specificity of the assay was evaluated using a double-blinded panel of 50 C. albicans strains. The assay showed a sensitivity of 96% and was able to detect all homozygous mutants included in the collection of strains, demonstrating that it is a robust, quick, and laborsaving method that is suitable for a routine clinical diagnostic laboratory.

Original language	English
Pages (from-to)	2037-2041
Number of pages	5
Journal	Journal of Clinical Microbiology
Volume	53
Issue number	7
DOIs	https://doi.org/10.1128/JCM.00398-15
State	Published - 1 Jul 2015

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title = "Quick detection of FKS1 mutations responsible for clinical echinocandin resistance in Candida albicans",

abstract = "A rapid molecular-based assay for the detection of the Candida albicans FKS1 gene mutations responsible for resistance to echinocandin drugs was designed and evaluated. The assay consisted of a multiplexed PCR set of 5 tubes able to detect the most commonly described resistance mechanism, including FKS1 hot spot 1 and hot spot 2 mutations. The performance and specificity of the assay was evaluated using a double-blinded panel of 50 C. albicans strains. The assay showed a sensitivity of 96\% and was able to detect all homozygous mutants included in the collection of strains, demonstrating that it is a robust, quick, and laborsaving method that is suitable for a routine clinical diagnostic laboratory.",

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AU - Dudiuk, Catiana

AU - Gamarra, Soledad

AU - Jimenez-Ortigosa, Cristina

AU - Leonardelli, Florencia

AU - Macedo, Daiana

AU - Perlin, David S.

AU - Garcia-Effron, Guillermo

PY - 2015/7/1

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Quick detection of FKS1 mutations responsible for clinical echinocandin resistance in Candida albicans

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